Research Articles
Endogenous Ubiquitin Western Blot: A Researcher's Guide to Eliminating Background Noise for Clear Results
This comprehensive guide addresses the critical challenge of high background noise in endogenous ubiquitin detection via western blot.
Endogenous Protein Extraction Without Deubiquitination: A Complete Guide for Research and Drug Development
This comprehensive article addresses the critical challenge of preserving endogenous ubiquitination states during protein extraction, a prerequisite for accurate signaling analysis and drug discovery.
Ubiquitination Signatures in Cancer: Prognostic Biomarkers and Therapeutic Targets
This article explores the rapidly evolving field of ubiquitination-based prognostic signatures in cancer.
Comparative Analysis of Ubiquitination Pathways in Cancer: From Molecular Mechanisms to Precision Therapeutics
Ubiquitination, a crucial post-translational modification, is profoundly dysregulated across cancer types, influencing tumorigenesis, progression, and therapeutic response.
Validating Ubiquitination Targets in Cancer vs. Normal Tissues: A Comprehensive Guide for Therapeutic Development
This article provides a systematic framework for researchers and drug development professionals on validating ubiquitination targets in cancer versus normal tissues.
Optimizing Proteasome Inhibition Assays in Cancer Cells: A 2025 Guide from Foundational Principles to Advanced Applications
This article provides a comprehensive guide for researchers and drug development professionals on optimizing proteasome inhibition assays in cancer models.
Troubleshooting E3 Ligase Functional Assays: A Comprehensive Guide from Validation to Optimization
This article provides a systematic framework for researchers, scientists, and drug development professionals to troubleshoot and optimize E3 ubiquitin ligase functional assays.
Improving Reproducibility in Ubiquitination Pathway Analysis: A Guide for Robust and Transparent Research
This article provides a comprehensive framework for researchers, scientists, and drug development professionals to enhance the reproducibility of their ubiquitination studies.
Overcoming Deubiquitinase Interference: A Methodological Guide for Accurate Assays and Therapeutic Discovery
Deubiquitinases (DUBs) are promising therapeutic targets, but experimental interference poses significant challenges in accurately characterizing their activity and substrate interactions.
Standardizing Ubiquitination Quantification: From Foundational Principles to Cross-Tissue Applications in Research and Drug Development
This article provides a comprehensive roadmap for researchers, scientists, and drug development professionals aiming to standardize the quantification of ubiquitination across diverse tissue types.