Research Articles
HECT vs. RING E3 Ubiquitin Ligases: Mechanisms, Regulation, and Therapeutic Targeting
This article provides a comprehensive comparison of HECT and RING E3 ubiquitin ligases, the key enzymes conferring specificity in the ubiquitin-proteasome system.
Ubiquitin and Ubiquitin-like Signaling: Evolutionary Origins, Mechanisms, and Pathogen Interactions in Prokaryotes vs. Eukaryotes
This article provides a comprehensive comparison of ubiquitin and ubiquitin-like (Ubl) protein conjugation systems across eukaryotes and prokaryotes.
Decoding the Ubiquitin Code: A Comprehensive Guide to Linkage-Specific Antibody Specificity, Applications, and Validation
This article provides a comprehensive resource for researchers and drug development professionals on linkage-specific ubiquitin antibodies, essential tools for cracking the complex 'ubiquitin code.' It covers the foundational biology of...
From APF-1 to Ubiquitin: The Convergent Discovery of a Central Regulatory Pathway in Cell Biology
This article explores the historical convergence of two independent research paths that identified the same fundamental protein—one as ATP-dependent proteolysis factor 1 (APF-1) in energy-dependent protein degradation studies, and the...
Validating Ubiquitin-Protein Isopeptide Linkage: Methods, Challenges, and Clinical Implications
This article provides a comprehensive overview of modern strategies for validating ubiquitin-protein isopeptide linkages, a critical process in understanding cellular regulation and disease mechanisms.
Ubiquitin Activation Across Kingdoms: From Evolutionary Origins to Therapeutic Targeting
This review provides a comprehensive analysis of the ubiquitin activation cascade across diverse species, from prokaryotic antecedents to complex eukaryotes.
K48 vs. K63 Polyubiquitin Chains: Decoding the Ubiquitin Code for Protein Fate and Signaling
This article provides a comprehensive comparison of K48- and K63-linked polyubiquitin chains, the two most abundant and functionally distinct ubiquitin codes.
Ubiquitin vs. SUMO: Decoding the Complex Crosstalk in Cellular Regulation and Therapeutic Targeting
This article provides a comprehensive comparison of the ubiquitin and SUMO post-translational modification pathways, essential regulatory systems in eukaryotic cells.
Validating Ubiquitin Conjugation ATP Dependence: From Molecular Mechanisms to Advanced Assay Design
This article provides a comprehensive guide for researchers and drug development professionals on validating the ATP dependence of protein ubiquitination.
APF-1 to Ubiquitin: Decoding the Seminal Discovery that Launched the Ubiquitin-Proteasome System
This article traces the pivotal discovery that ATP-dependent proteolysis factor 1 (APF-1) is identical to the protein ubiquitin, a breakthrough that unified disparate lines of biological research and laid the...