This article provides researchers, scientists, and drug development professionals with a complete methodological framework for preserving labile ubiquitin modifications during immunoprecipitation experiments.

This article provides a comprehensive comparison between ubiquitinomics and proteomics for researchers and drug development professionals engaged in cancer biomarker discovery.

This article provides a comprehensive synthesis of ubiquitin-like proteins (UBLs), exploring their conserved β-grasp fold structure, intricate enzymatic conjugation cascades, and profound evolutionary lineage tracing back to prokaryotic sulfur-transfer systems.

This article provides a definitive, step-by-step guide for researchers and drug development professionals on reliably detecting protein ubiquitination via immunoblotting.

This article provides a comprehensive guide for researchers and drug development professionals on the use and optimization of the deubiquitylase (DUB) inhibitors N-Ethylmaleimide (NEM) and Iodoacetamide (IAA).

This comprehensive review synthesizes current knowledge on the systematic alterations in ubiquitination patterns between cancerous and normal tissues.

This article provides a comprehensive resource for researchers on Ubiquitin-Binding Domain (UBD)-based affinity enrichment, a cornerstone technique for profiling the ubiquitinome.

This article provides a detailed overview of Tandem Ubiquitin-Binding Entities (TUBEs), powerful molecular tools designed to overcome long-standing challenges in ubiquitin research.

The Ubiquiton system represents a transformative synthetic biology tool that enables rapid, inducible, and linkage-specific polyubiquitylation of proteins of interest in living cells.

This article provides a comprehensive guide for researchers and drug development professionals facing challenges in identifying protein ubiquitination sites using mass spectrometry.

This article provides researchers, scientists, and drug development professionals with a comprehensive guide to the theory and application of deubiquitinase (DUB)-based analysis for validating ubiquitin chain architecture.

This article provides a comprehensive guide for researchers analyzing ubiquitinated proteins, detailing how strategic selection of SDS-PAGE running buffers—MES, MOPS, and Tris-acetate—significantly impacts the resolution of different ubiquitin chain lengths...