Research Articles
Ubiquitination-Related Gene Signatures: Novel Prognostic Biomarkers and Therapeutic Guides in Cancer
Ubiquitination, a crucial post-translational modification, is increasingly recognized for its role in tumorigenesis, progression, and therapy response.
A Comprehensive Guide to Immunoblotting for Ubiquitinated Proteins: Best Practices from Sample Preparation to Data Interpretation
This article provides a definitive, step-by-step guide for researchers and drug development professionals on reliably detecting protein ubiquitination via immunoblotting.
Optimizing NEM and IAA Concentration for Deubiquitylase (DUB) Inhibition: A Guide for Experimental Design and Validation
This article provides a comprehensive guide for researchers and drug development professionals on the use and optimization of the deubiquitylase (DUB) inhibitors N-Ethylmaleimide (NEM) and Iodoacetamide (IAA).
Optimizing SDS-PAGE Buffer Selection: A Guide to MES, MOPS, and Tris-Acetate for Ubiquitin Chain Resolution
This article provides a comprehensive guide for researchers analyzing ubiquitinated proteins, detailing how strategic selection of SDS-PAGE running buffers—MES, MOPS, and Tris-acetate—significantly impacts the resolution of different ubiquitin chain lengths...
Ubiquiton: Mastering Linkage-Specific Polyubiquitylation to Decode Cellular Signals and Advance Therapeutics
The Ubiquiton system represents a transformative synthetic biology tool that enables rapid, inducible, and linkage-specific polyubiquitylation of proteins of interest in living cells.
His-Tag vs. Strep-Tag Ubiquitin Purification: A Comprehensive Guide from Principles to Practice
This article provides researchers, scientists, and drug development professionals with a definitive guide to purifying ubiquitinated proteins using His-tag and Strep-tag affinity systems.
Linkage-Specific Ubiquitin Antibodies: A Comprehensive Guide for Immunoblotting Applications in Research and Drug Development
This article provides a comprehensive resource for researchers and drug development professionals utilizing linkage-specific ubiquitin antibodies in immunoblotting.
TUBE Technology: A Comprehensive Guide to Isolating and Studying Ubiquitinated Proteins
This article provides a detailed overview of Tandem Ubiquitin-Binding Entities (TUBEs), powerful molecular tools designed to overcome long-standing challenges in ubiquitin research.
Mass Spectrometry-Based Ubiquitinomics: Advanced Proteomic Approaches for Biomarker Discovery and Drug Development
This article provides a comprehensive overview of mass spectrometry (MS)-based ubiquitinomics, a specialized field of proteomics dedicated to profiling protein ubiquitination.
UBB and UBC: Decoding Polyubiquitin Gene Organization, Regulation, and Therapeutic Targeting
This article provides a comprehensive analysis of the polyubiquitin genes UBB and UBC, which are critical for maintaining cellular ubiquitin homeostasis.