Research Articles
Troubleshooting Ubiquitination Site Identification by Mass Spectrometry: Strategies to Overcome Key Challenges
This article provides a comprehensive guide for researchers and drug development professionals facing challenges in identifying protein ubiquitination sites using mass spectrometry.
Optimizing Ubiquitin Chain Resolution: A Practical Guide to Gel Percentage and Buffer Selection for Researchers
This article provides a comprehensive, practical guide for researchers and drug development professionals aiming to optimize the separation and analysis of ubiquitin chains via SDS-PAGE.
Preserving Labile Ubiquitin Chains: A Complete Guide to Stabilizing K63 and M1 Linkages for Accurate Analysis
This comprehensive guide addresses the critical challenge of preserving labile K63- and M1-linked ubiquitin chains during biological sample preparation.
Overcoming Low Stoichiometry: Advanced Strategies for Ubiquitination Detection in Biomedical Research
This comprehensive review addresses the significant analytical challenge of detecting protein ubiquitination at low stoichiometry, a fundamental post-translational modification with critical roles in cellular regulation and disease pathogenesis.
Optimized Western Blotting for High Molecular Weight Ubiquitinated Proteins: A Complete Guide from Theory to Validation
This article provides a comprehensive guide for researchers and drug development professionals aiming to reliably detect high molecular weight ubiquitinated proteins via immunoblotting.
Strategies to Reduce Non-Specific Binding in Ubiquitinated Protein Enrichment: A Guide for Reliable Proteomics
This article provides a comprehensive guide for researchers and drug development professionals seeking to optimize the enrichment of ubiquitinated proteins for proteomic analysis.
A Comprehensive Guide to Preventing Deubiquitination During Cell Lysis: Strategies for DUB Inhibitor Implementation in Proteostasis Research
This article provides researchers, scientists, and drug development professionals with a comprehensive framework for preserving cellular ubiquitination states during protein extraction.
MG132 Proteasome Inhibition: Decoding Cytotoxicity Through Treatment Duration and Dose Optimization
This comprehensive review examines the complex relationship between MG132 treatment duration, concentration, and cytotoxic outcomes across diverse cancer models.
Decoding the Ubiquitinome: A Comprehensive Guide to UBD-Based Affinity Enrichment Methods
This article provides a comprehensive resource for researchers on Ubiquitin-Binding Domain (UBD)-based affinity enrichment, a cornerstone technique for profiling the ubiquitinome.
Optimizing N-Ethylmaleimide (NEM) Concentration for Effective DUB Inhibition: A Guide for Researchers
This article provides a comprehensive guide for researchers and drug development professionals on optimizing N-Ethylmaleimide (NEM) concentration for effective deubiquitylating enzyme (DUB) inhibition.