Research Articles
Deubiquitinating Enzymes (DUBs) in Cancer: Mechanisms, Therapeutic Targeting, and Future Directions
This comprehensive review elucidates the critical functions of deubiquitinating enzymes (DUBs) in oncogenesis and cancer progression.
E3 Ubiquitin Ligases in Tumorigenesis: Molecular Mechanisms, Therapeutic Targeting, and Future Directions
This review comprehensively examines the critical roles of E3 ubiquitin ligases in cancer development and progression.
In Vivo vs. In Vitro Ubiquitination Detection: A Comprehensive Guide for Researchers
This article provides a detailed comparison of in vivo and in vitro ubiquitination detection techniques, tailored for researchers and drug development professionals.
Bridging Discovery and Function: A Practical Guide to Correlating Ubiquitination MS Data with Functional Assays
This article provides a comprehensive roadmap for researchers and drug development professionals aiming to move beyond the simple identification of ubiquitination sites and toward a functional understanding of this critical...
UBD vs. Antibody-Based Methods: A Comprehensive Guide to Evaluating Enrichment Efficiency for Protein Ubiquitination
This article provides a systematic evaluation of Ubiquitin-Binding Domain (UBD)-based and antibody-based methods for enriching ubiquitinated proteins, a critical step in proteomics and drug discovery.
Benchmarking Mass Spectrometry Sensitivity for diGly Peptide Detection: Advances in DIA, Rescoring, and Deep Ubiquitinome Analysis
This article provides a comprehensive benchmark of mass spectrometry platforms and methodologies for the sensitive detection of endogenously ubiquitinated diGly peptides.
Decoding the Ubiquitin Code: A 2025 Researcher's Guide to Linkage-Specific Antibody Performance
This article provides a comprehensive comparison of ubiquitin linkage-specific antibody performance for researchers and drug development professionals.
Quantitative Accuracy in Ubiquitination Profiling: A Comprehensive Comparison of SILAC vs. Label-Free Proteomics
This article provides a systematic assessment of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and label-free quantitative proteomics for ubiquitination analysis, a critical post-translational modification.
Ubiquitin Tagging vs. Antibody Enrichment: A Strategic Guide for Proteomics and Therapeutic Development
This article provides a comprehensive comparison of two pivotal methods for analyzing protein ubiquitination: ubiquitin tagging and antibody-based enrichment.
DIA vs DDA for Ubiquitinome Analysis: A Comprehensive Comparison for Proteomics Researchers
This article provides a systematic comparison of Data-Dependent Acquisition (DDA) and Data-Independent Acquisition (DIA) methodologies for ubiquitinome analysis, addressing the critical need for optimized workflows in proteomics research.