Research Articles
Preserving the Ubiquitin Code: A Comprehensive Guide to Preventing Deubiquitination During Protein Extraction
This article provides a definitive guide for researchers and drug development professionals on preventing deubiquitination during protein extraction.
Optimizing Ubiquitin Chain Binding Capacity Assays: A Guide to Methods, Troubleshooting, and Validation
This article provides a comprehensive guide for researchers and drug development professionals aiming to optimize assays for characterizing ubiquitin chain binding interactions.
Advanced Strategies to Resolve Ubiquitin Smears in Western Blotting: A Guide for Protein Researchers
This article provides a comprehensive guide for researchers and drug development professionals seeking to resolve the challenging ubiquitin smears in Western blots.
Overcoming E3 Ligase Specificity Challenges: From Degron Mapping to Precision Therapeutics
This article addresses the central challenge in targeted protein degradation: harnessing the specificity of over 600 human E3 ubiquitin ligases for therapeutic applications.
Advanced Methods for Enriching Low-Abundance Ubiquitinated Proteins: From Fundamental Principles to Clinical Applications
This comprehensive review explores cutting-edge methodologies for enriching low-abundance ubiquitinated proteins, a critical challenge in proteomics and drug discovery.
Bridging the Gap: A Researcher's Guide to Artifacts in Ubiquitin Detection and Validation
Accurate detection and quantification of ubiquitination are paramount for advancing our understanding of this crucial post-translational modification in health and disease.
Ubiquitin Immunoprecipitation Background Reduction: A Strategic Guide for Cleaner Pull-Downs and Reliable Data
This article provides a comprehensive framework for researchers and drug development professionals to systematically minimize background in ubiquitin immunoprecipitation (IP) experiments.
Optimizing MG-132 Treatment: A Strategic Guide to Time and Concentration for Cancer Research
This article provides a comprehensive guide for researchers and drug development professionals on optimizing the proteasome inhibitor MG-132, a critical tool compound and precursor to clinical agents.
Overcoming Weak Immunogenicity in Ubiquitin Antibodies: Advanced Strategies for Research and Therapeutic Development
This article addresses the significant challenge of weak immunogenicity in ubiquitin antibodies, a major bottleneck in proteomics and therapeutic development.
Beyond Lysine: A Comprehensive Guide to Non-Canonical Ubiquitination Detection Methods
This article provides a comprehensive overview of the rapidly evolving field of non-canonical ubiquitination detection.